Wnt10b promotes hair follicles growth and dermal papilla cells proliferation via Wnt/β-Catenin signaling pathway in Rex rabbits.

Hongli Liu,Zhenyu Wu,Gongyan Liu,Fuchang Li,Yanli Zhu

doi:10.1042/bsr20191248

Abstract

Wnt signaling plays an important role in the growth and development of hair follicles (HFs). Among the signaling molecules, Wnt10b was shown to promote the differentiation of primary skin epithelial cells toward the hair shaft and inner root sheath of the HF cells in mice in vitro. Whisker HFs were isolated from Rex rabbits and cultured in vitro to measure hair shaft growth. Meanwhile, dermal papilla cells (DPCs) were isolated and cultured in vitro. Treatment with AdWnt10b or the Wnt/β-Catenin Pathway inhibitor, XAV939, assessed the DPCs proliferation by CCK-8 assay. And the cell cycle was also analyzed by flow cytometry. We found that Wnt10b could promote elongation of the hair shaft, whereas XAV-939 treatment could eliminated this phenomenon. AdWnt10b treatment promoted the proliferation and induced G1/S transition of DPCs. AdWnt10b stimulation up-regulated β-Catenin protein in DPCs. Inhibition of Wnt/β-Catenin signaling by XAV-939 could decreased the basal and Wnt10b-enhanced proliferation of DPCs. And could also suppress the cell cycle progression in DPCs. In summary, our study demonstrates that Wnt10b could promote HFs growth and proliferation of DPCs via the Wnt/β-Catenin signaling pathway in Rex rabbits.

Highlights

Hair follicles (HFs) contain dermal papilla cells (DPCs) and epithelial cells, which are the two major types of cell [1]
To understand the role of Wnt10b in the HFs of Rex rabbits, the present study investigated the effects of inhibition and activation of the Wnt/β-Catenin signaling pathway in HF shafts growth and the DPCs proliferation from Rex rabbits
Isolated Rex rabbit whisker HFs at early or mid-anagen were cultured for 5 days with AdWnt10b, AdGFP or the basal medium

Summary

Introduction

Hair follicles (HFs) contain dermal papilla cells (DPCs) and epithelial cells, which are the two major types of cell [1]. Formation of new DP can be induced in adult skin by activating the Wnt pathway in the epidermis [4]. Several families of secreted signaling molecules have been implicated in the communication between the epidermis and dermis during HF development [6]. Among these processes, Wnt/β-Catenin signaling has been shown to be required for the initiation and regeneration of HF development in mice [7]. In the Wnt/β-Catenin signaling pathway, β-Catenin takes over an important role in the formtion of HFs. When β-Catenin accumulates in the cytoplasm, it can be translocated to the nucleus, where it interacts with the TCF/LEF transcription factors to activate gene expression [8]. The formation of placodes that generate HF is blocked if the β-Catenin is mutated during embryogenesis [9]

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Conclusion