Abstract

The document associated with this DOI has been withdrawn.

Highlights

  • Direct RNA nanopore sequencing of SARS-CoV-2 extracted from critical material from swabs

  • We identify COVID-19 sequences by direct-RNA-seq protocol, means of Oxford Nanopore MinION technology, from both high fragmentation and very low concentration RNA, which is directly extracted from the routine oropharyngeal swab

  • In consideration of the increasing prevalence of COVID-19 cases in several countries and the resulting demand for unbiased sequencing approaches, we performed a direct RNA sequencing experiment using critical oropharyngeal swab samples collected from Italian patients infected with SARS-CoV-2 from the Palermo region in Sicily

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Summary

Introduction

In consideration of the increasing prevalence of COVID-19 cases in several countries and the resulting demand for unbiased sequencing approaches, we performed a direct RNA sequencing (direct RNA seq.) experiment using critical oropharyngeal swab samples collected from Italian patients infected with SARS-CoV-2 from the Palermo region in Sicily. Direct RNA nanopore sequencing of SARS-CoV-2 extracted from critical material from swabs

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