Abstract
// Jiao Feng 1, * , Zhe Yin 1, * , Zhe Zhan 1 , Haifeng Mao 2 , Xiaoyuan Jiang 1 , Lijun Zeng 1 , Wenhui Yang 1 , Huiying Yang 1 , Jinglin Wang 1 , Haijian Zhou 3, 4 and Dongsheng Zhou 1 1 State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing, 100071, China 2 The First People’s Hospital of Lianyungang, Lianyungang, 222002, China 3 State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, 102206, China 4 Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Hangzhou, 310003, China * These authors contributed equally to this work Correspondence to: Dongsheng Zhou, email: dongshengzhou1977@gmail.com Haijian Zhou, email: zhouhaijian@icdc.cn Jinglin Wang, email: wjlwjl0801@sina.com Keywords: Klebsiella pneumoniae; multidrug resistance; plasmids; p675920-1 Received: July 10, 2017 Accepted: December 05, 2017 Published: January 13, 2018 ABSTRACT This study dealt with detailed genomic characterization of two multidrug resistant (MDR) plasmids p675920-1 and p675920-2 from a single clinical Klebsiella pneumoniae isolate 675920. p675920-1 was essentially a hybrid of the IncFII plasmid pHN7A8 and the IncR plasmid pKPC-LK30, and functioned as an IncFII plasmid with inactivation of the IncR replication gene. The backbone of p675920-2 was a hybrid of a novel replicon, three maintenance regions (22.0-, 2.7-, 2.6-kb in length, respectively) as found in pKPYL2, p10164-3 and pK1HV, respectively, and the entire 25.9-kb conjugal transfer region of pKPYL2. p675920-1 and p675920-2 carried a large number of resistance genes, which contributed to resistance to at least seven classes of antibiotics (β-lactams, quinolones, aminoglycosides, fosfomycins, sulphonamides, trimethoprims, and tetracyclines) and one kind of heavy mental (mercury). All of these resistance genes are associated with mobile elements such as insertion sequences, insertion sequence-based transposition units, and transposons, which constituted a total of three novel MDR regions, two in p675920-1 and another in p675920-2. Coexistence of two MDR plasmids p675920-1 and p675920-2 made K. pneumoniae 675920 tend to become extensively drug-resistant.
Highlights
Plasmids of the incompatibility group IncFII are usually low copy number plasmids (> 100 kb in size) with a narrow host range and circulated mainly among Enterobacteriaceae species [1]
The modular structure of each plasmid was divided into the backbone regions and the separate accessory modules, which were defined as acquired DNA regions associated with and bordered by mobile elements and inserted at different sites of the backbone (Figure 1, Supplementary Figure 1 and Supplementary Table 1)
A total of ten non-redundant genes or gene loci (blaKPC-2, blaCTX-M-65, blaTEM-1, blaLAP-2, qnrS1, rmtB, tetA(A), sul2, fosA3, and the mer locus), which were involved in resistance to antimicrobials and heavy metal, were found in the accessory modules of these two plasmids (Supplementary Table 1 and Supplementary Table 2)
Summary
Plasmids of the incompatibility group IncFII are usually low copy number plasmids (> 100 kb in size) with a narrow host range and circulated mainly among Enterobacteriaceae species [1]. All of resistance genes (blaCTX-M-65, blaTEM-1, rmtB, and fosA3) of this plasmid are harbored within a 14.5-kb MDR region, which is the sole accessory module of pHN7A8 and inserted at a site downstream of pemIK. These resistance genes are associated with mobile elements including one copy of Tn2, IS1, ISEcp, IS1294, and IS903D and four copies of IS26, which denotes a complex history of transposition and homologous recombination
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
