WITHDRAWN: Highly sensitive and specific resonance Rayleigh scattering detection of esophageal cancer cells via dual-aptamer target binding strategy

Abstract

In this work, EGFR aptamer (Apt 1) and HER2 aptamer (Apt 2) were modified with gold nanoparticles (AuNPs) to obtain Probe I (Apt 1-AuNPs) and Probe II (Apt 2-AuNPs). Taking Eca109, KYSE510 and KYSE150 cells as models, the sandwich scattering system of probe I -cell-probe II was formed by the recognition of tumor markers by the aptamer modified probe, and the resonance Rayleigh scattering (RRS) spectra were investigated. The results showed that the scattering system can be used to quantitatively detect the above three cell lines with detection limits of 15 cells·mL-1 (Eca109), 18 cells·mL-1 (KYSE510), and 12 cells·mL-1 (KYSE150). Based on the probe I -cell-probe II sandwich strategy, a dual probe RRS method for the quantitative determination of esophageal cancer cells was established.