A Highly Sensitive Resonance Rayleigh Scattering Method for the Determination of Vitamin B1 with Gold Nanoparticles Probe

Abstract

In weakly acidic buffer medium, vitamin B1 (VB1) interacts with gold nanoparticles to form a binding product, which resulted in a significant enhancement of resonance Rayleigh scattering (RRS) intensity and the appearance of a new RRS spectrum. The maximum RRS peak was at 368 nm, and there are three smaller scattering peaks that were at 284 nm, 440 nm and 495 nm, respectively. The enhanced RRS intensity (ΔI) was directly proportional to the concentration of VB1 in the range of 0–2.8 × 10−7 mol L−1. The method had high sensitivity and its detection limit (3σ) was 0.9 ng mL−1. The optimum conditions and the influencing factors have been investigated. The method had good selectivity, which could be observed from the influence of coexisting substances. A sensitive, simple and fast RRS method for the determination of VB1 with gold nanoparticle probe has been developed. In addition, the reasons for RRS enhancement were discussed.