Abstract
Hirschsprung-associated enterocolitis (HAEC) stands as most common and serious complication of Hirschsprung disease. Variations in the microbiota composition may account for the differences observed between HAEC and healthy individuals, offering crucial insights into the disease's pathogenesis. Here, we performed a study to Changes in the gut microbiome using 16sRNA amplicon sequencing in a cohort of HAEC patients (n=16) and healthy controls (n=14). Our result revealed a significant disparity in beta diversity between the two groups. Following correction for false discovery rate (FDR), a rank-sum test at the genus level indicated a notable decrease in the relative abundance of Bifidobacterium, Lactobacillus, and Veillonella, whereas the Enterococcus genus exhibited a substantial increase in HAEC, a finding further supported by additional LEfSe analysis. Functional analysis showed that putative transport and catabolism, Digestive system, and metabolism of cofactors and vitamins were proved to be some abundant KOs in healthy group, while Infectious disease, Membrane transport, Carbohydrate metabolism were the 3 KOs with the higher abundance in the HAEC group. Our data increased our insight into the HAEC, which may shed further light on HAEC pathogenesis. Our study firstly demonstrated the difference between fecal microbiota of HAEC patients and healthy individuals, which made a step forward in the understanding of the pathophysiology of Hirschsprung-associated enterocolitis.
Published Version
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