Wistar Ratlarında Dietilnitrozamin Uygulanarak Oluşturulan Hepatoselüler Karsinom Modelinde Silimarin’in Koruyucu Etkisi; Kanser Paternleri ve Sitolojik Varyantlar

Abstract

In this study, the protective effect of silymarin was investigated with histopathological methods in terms of cancer patterns and cytological variants in the hepatocellular carcinoma model created by applying diethylnitrosamine experimentally in rats. For this purpose, the rats were randomly divided into 5 groups. The rats in the control group were fed pellet feed and drinking water for 20 weeks. Animals in Sham group were administered with propylene glycol dissolved in 0.9% NaCl intraperitoneally 3 times a week for 20 weeks. Animals in DEN group administered DEN at a dose of 50 mg/kg once a week for 20 weeks. The rats in the DEN +Silymarin group were Silymarin intraperitoneally administered for 21 weeks 3 times a week at a dose of 100 mg/kg, starting 1 week prior to the administration of DEN. Silymarin was administered to rats in the group of Silymarin 3 times a week intraperitoneally for 20 weeks at a dose of 100 mg/kg. At the end of the study, systemic necropsy of the animals was performed and liver tissue samples were taken. After routine tissue procedures, sections were examined under the light microscope. We observed that Hepatocellular carcinoma occurred in DEN and DEN + Silymarin groups.