Abstract
A Love wave-based biosensor with a 440 MHz center frequency was developed for the simultaneous detection of two different analytes of Cartilage Oligomeric Matrix Protein (COMP) and rabbit immunoglobulin G (IgG) in a single sensor. The developed biosensor consists of one-port surface acoustic wave (SAW) reflective delay lines on a 41° YX LiNbO3 piezoelectric substrate, a poly(methyl methacrylate) (PMMA) waveguide layer, and two different sensitive films. The Love wave biosensor was wirelessly characterized using two antennas and a network analyzer. The binding of the analytes to the sensitive layers induced a large change in the time positions of the original reflection peaks mainly due to the mass loading effect. The assessed time shifts in the reflection peaks were matched well with the predicted values from coupling of mode (COM) modeling. The sensitivities evaluated from the sensitive films were ∼15 deg/μg/mL for the rabbit IgG and ∼1.8 deg/ng/mL for COMP.
Highlights
In recent years, there has been increasing interest in shear horizontal surface acoustic wave (SH-SAW)-based biosensors for detecting bioanalyte types and concentrations because of their low signal attenuation in liquids, fast response time, high sensitivity, the direct use of raw biological samples for detection, label-free assay, and the simple implementation of array systems
We introduce a new type of wireless, battery-free Love wave-based biosensor that utilizes a one-port SAW reflective delay line for the simultaneous measurement of two different biomolecules in a single device
The IDT and shorted grating reflectors were placed onto the 41° YX LiNbO3 substrate for the SAW reflective delay line
Summary
There has been increasing interest in shear horizontal surface acoustic wave (SH-SAW)-based biosensors for detecting bioanalyte types and concentrations because of their low signal attenuation in liquids, fast response time, high sensitivity, the direct use of raw biological samples for detection, label-free assay, and the simple implementation of array systems. The frequencies of the two SAW devices are mixed to provide a frequency difference between the two that is directly proportional to the target biomolecule concentration By using this dual track delay line configuration, SAW Instruments GmbH We introduce a new type of wireless, battery-free Love wave-based biosensor that utilizes a one-port SAW reflective delay line for the simultaneous measurement of two different biomolecules in a single device. Compared with other currently available wired biosensors, this sensor offers several advantages: no battery installation, absence of a complicated wireless communication circuitry, simultaneous measurement of multiple biocells by a single device, high sensitivity, good protection of the IDT and reflector from liquid environment, and simple temperature compensation. Using the extracted optimal design parameters, the device was fabricated and wirelessly characterized under different antigen concentrations
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