Abstract
SPAD value was measured by a portable chlorophyll instrument, which can reflect the relative chlorophyll content of vegetation well. Chlorophyll is an important organic chemical substance in plants that acquires and transmits energy during photosynthesis. The continuous spectral curve of winter wheat can be obtained rapidly in a specific band range by using hyperspectral remote sensing technology to estimate the SPAD value of winter wheat, which is of great significance to the growth monitoring and yield estimation research of winter wheat. In this study, with winter wheat as the research object, the spectral data and corresponding SPAD value in different growth stages were used as the data source, 20 kinds of data preprocessing spectra and sensitive spectral indices set the data as model input values, the partial least square regression (PLSR) model was established to estimate the SPAD value, and the model estimation results of different model input values at different growth stages were compared in detail. The results showed that the set of sensitive spectral indices selected in this study as input values can effectively improve the accuracy and stability of the PLSR model. In addition, the effects of 20 spectral data pretreatment methods on the estimation results of the SPAD value were compared and analyzed in different growth stages. It was found that the spectral data pretreated by the combination of wavelet packet denoising, first-order derivative transformation and principal component analysis can improve the accuracy and stability of PLSR model, and it is suitable for all growth stages. The results also showed that the estimation model is highly sensitive to the standard deviation of the SPAD value (STDchl) in sample sets. When the standard deviation is greater than 5.5 SPAD, the larger the STDchl is, the higher the model estimation accuracy is, and the more stable the model is. At this time, the model estimation accuracy is higher (R2V is greater than 0.5, ratio of performance to deviation is greater than 1.4), which can meet the estimation requirements of the SPAD value.
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