Wild-type α-synuclein interacts with pro-apoptotic proteins PKCδ and BAD to protect dopaminergic neuronal cells against MPP +-induced apoptotic cell death

Abstract

α-Synuclein is a pre-synaptic protein of unknown function that has been implicated in the pathogenesis of Parkinson's disease (PD). Recently, we demonstrated that 1-methyl-4-phenylpyridinium (MPP +) induces caspase-3-dependent proteolytic activation of PKCδ, which subsequently contributes to neuronal apoptotic cell death in mesencephalic dopaminergic neuronal cells [50,96]. In the present study, we examined whether PKCδ interacts with α-synuclein to modulate MPP +-induced dopaminergic degeneration. Over-expression of wild-type human α-synuclein in mesencephalic dopaminergic neuronal cells (N27 cells) attenuated MPP +-induced (300 μM) cytotoxicity, release of mitochondrial cytochrome c, and subsequent caspase-3 activation, without affecting reactive oxygen species (ROS) generation. Wild-type α-synuclein over-expression also dramatically reduced MPP +-induced caspase-3-mediated proteolytic cleavage of PKCδ, whereas over-expression of the mutant human α-synuclein A53T did not alter the PKCδ cleavage under similar conditions. Immunoprecipitation-kinase assay revealed reduced PKCδ kinase activity in wild-type α-synuclein over-expressing cells in response to MPP + treatment. Wild-type α-synuclein over-expression also rescued mesencephalic dopaminergic neuronal cells from MPP +-induced apoptotic cell death, while α-synuclein A53T exacerbated the MPP +-induced DNA fragmentation. Furthermore, co-immunoprecipitation studies revealed that α-synuclein interacts with the pro-apoptotic proteins PKCδ and BAD, but not with the anti-apoptotic protein Bcl-2 following MPP + treatment. We also observed that the interaction between PKCδ and α-synuclein does not involve direct phosphorylation. Together, our results demonstrate that wild-type α-synuclein interacts with the pro-apoptotic molecules BAD and PKCδ to protect dopaminergic neuronal cells against neurotoxic insults.