Abstract
Abstract The Janetschek method is a variation of the simple frequency method and therefore subject to the same limitations. Instars can only be reliably determined by these methods if development within the population is known to be homogeneous. The number of nymphal instars proposed by Kondratieff and Voshell (1980) for an overwintering population of Stenonema modestum in the North Anna River of Virginia is not substantiated. These authors estimated a total of 14–15 nymphal instars using the simple frequency and Janetschek methods. A reexamination of their paper indicates that the development of this 5. modestum population is quite variable and therefore not amenable to successful instar determination using the simple frequency, Janetschek or any other indirect instar determination method. Indirect instar determination methods cannot be considered reliable in determining the number of instars of Ephemeroptera because of the developmental variability characteristic of most, if not all, mayfly species.
Published Version
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