Why chartreuse? The pigment vulpinic acid screens blue light in the lichen Letharia vulpina.

Abstract

Chlorophyll fluorescence, infrared gas exchange and photoinhibition data consistently show that vulpinic acid in L. vulpina functions as a strong blue lightscreening compound. The cortical lichen compounds, parietin, atranorin, usnic acid and melanins are known to screen photosynthetically active radiation (PAR), thereby protecting the underlying photobionts. The role of the toxic UV-/blue light-absorbing vulpinic acid in lichen cortices is poorly documented. By comparing controls with acetone-rinsed Letharia vulpina thalli (75% reduced vulpinic acid concentration), we aimed to test PAR screening by vulpinic acid. We exposed such thalli to blue, green and red irradiance, respectively, and recorded light quality-specific light saturationcurves of CO2 uptake, quantum yields of CO2 uptake (QYCO2) and effective quantum yields of PSII (ΦPSII). We also quantified light quality-dependent photoinhibition after 4-h exposure to 400µmol photons m-2s-1. In controls, the greatest high light-induced reductions in CO2 uptake and ΦPSII, as well as the strongest photoinhibition [lowered maximal quantum yield of PSII (Fv/Fm)], occurred in red light, followed by green, and was low in blue light. Removal of vulpinic acid significantly exacerbated photoinhibition, reduced ΦPSII, and increased QYCO2 in blue light. By contrast, acetone rinsing had no or weak effects in green and red lights. Comparing control with acetone-rinsed thalli, blue light screening was estimated at 69% using ΦPSII data and 49% using QYCO2. To compensate for the 25% residual vulpinic acid left after rinsing, we repeated the screening estimation by comparing responses in blue and red lights. This resulted in 88% screening using ΦPSII data and 77% using QYCO2. The consistent responses in all photosynthetic parameters support the hypothesis that vulpinic acid functions as a blue light screen in L. vulpina.