Abstract
Phormium tenax is a kind of drought resistant garden plant with its rich and colorful leaves. To clarify the molecular mechanism of drought resistance in Phormium tenax, transcriptome was sequenced by the Illumina sequencing technology under normal and drought stress, respectively. A large number of contigs, transcripts and unigenes were obtained. Among them, only 30,814 unigenes were annotated by comparing with the protein databases. A total of 4,380 genes were differentially expressed, 2,698 of which were finally annotated under drought stress. Differentially expression analysis was also performed upon drought treatment. In KEGG pathway, the mechanism of drought resistance in Phormium tenax was explained from three aspects of metabolism and signaling of hormones, osmotic adjustment and reactive oxygen species metabolism. These results are helpful to understand the drought tolerance mechanism of Phormium tenax and will provide a precious genetic resource for drought-resistant vegetation breeding and research.
Highlights
The results showed that Phormium tenax under drought stress has longer taproot and lateral root compared to those grown in normal condition, while the shoot height was opposite
There has been a slight increase in the seven physiological characteristics during drought stress in Phormium tenax (Figure S1)
According to Gene Ontology (GO) annotation with a total number of 1,438, we found that biological processes (BP), cellular component (CC) and molecular function (MF), respectively, accounted for 45.04%, 34.36% and 20.63% (Figure S6)
Summary
Plant materials and stress treatment.Pot and artificial water control method were used to simulate the drought. Put the soil into the same size of plastic basins, 3 kg per pot, a total of 40 pots. The water content of the soil was controlled by 70% of the saturated water content of the soil (after the experiment, the saturated water content of the soil was 65.04%). One-year-old Phormium tenax which have consistent height and biomass were selected as materials and transplanted to plastic basins, 1 pot per pot. Watering according to the plant’s growth, the spilled water was poured back into the basin in order to maintain the soil moisture consistent. After 30 days of growth, 40 pot seedlings were randomly divided into two groups: control group (T02) and experimental group (T01). Soil water content was measured every 2 days to replenish the amount of deficiency. Phormium tenax with different treatment were harvested for further use
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
