Abstract
Artemia is an industrially important genus used in aquaculture as a nutritious diet for fish and as an aquatic model organism for toxicity tests. However, despite the significance of Artemia, genomic research remains incomplete and knowledge on its genomic characteristics is insufficient. In particular, Artemia franciscana of North America has been widely used in fisheries of other continents, resulting in invasion of native species. Therefore, studies on population genetics and molecular marker development as well as morphological analyses are required to investigate its population structure and to discriminate closely related species. Here, we used the Illumina Hi-Seq platform to estimate the genomic characteristics of A. franciscana through genome survey sequencing (GSS). Further, simple sequence repeat (SSR) loci were identified for microsatellite marker development. The predicted genome size was ∼867 Mb using K-mer (a sequence of k characters in a string) analysis (K = 17), and heterozygosity and duplication rates were 0.655 and 0.809%, respectively. A total of 421467 SSRs were identified from the genome survey assembly, most of which were dinucleotide motifs with a frequency of 77.22%. The present study will be a useful basis in genomic and genetic research for A. franciscana.
Highlights
Received: 17 November 2020Revised: 17 January 2021Accepted: 23 February 2021Accepted Manuscript online: Version of Record published: The genus Artemia (Crustacea: Branchiopoda: Anostraca), known as brine shrimp, is an aquatic invertebrate living mainly in salt lakes
They are widely used as aquatic model organisms for ecotoxicity tests, along with Daphnia [4–6]
A total of 22.8 Gb of raw data for A. franciscana were generated by Illumina paired-end library (Table 1)
Summary
Accepted Manuscript online: Version of Record published: The genus Artemia (Crustacea: Branchiopoda: Anostraca), known as brine shrimp, is an aquatic invertebrate living mainly in salt lakes. Artemia species are important in aquaculture industry because their dormant cysts are hatched, and the nauplii can be used as a nutrient-rich food for fish [2,3]. They are widely used as aquatic model organisms for ecotoxicity tests, along with Daphnia [4–6]. Despite the significance of Artemia in aquaculture, genomic research is still incomplete, and the genomic characteristics are less known, compared with. A native species in North America, has been extensively used and introduced to other continents for commercial purposes, thereby affecting the local population’s biodiversity [10,11]
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.