Whole-Genome Sequence of Rickettsia parkeri Strain Atlantic Rainforest, Isolated from a Colombian Tick

Andrés F Londoño,David H Walker,Juan D Rodas,Donald H Bouyer,Thomas G Wood,Andrew L Routh,Steven G Widen,Nicole L Mendell,Gustavo A Valbuena,Julie C Dunning Hotopp

doi:10.1128/mra.00684-19

Abstract

Rickettsia parkeri is classified as a member of the alphaproteobacterial microorganisms, genus Rickettsia Here, we report the complete genome sequence of Rickettsia parkeri strain Atlantic Rainforest, which was isolated from an Amblyomma ovale tick collected in the municipality of Necoclí, Colombia.

Highlights

Rickettsia parkeri, which is an obligately intracellular bacterium, has across the American continents as infecting different tick species been reported and has been associated with a relatively mild human disease [1]
6-well plate containing Vero cell monolayers, and the plate was incubated at 34°C in an atmosphere of 5% CO2 for 1 hour
In week 3, when rickettsiae were detected, isolation was confirmed by PCR [2, 4]

Summary

Introduction

Rickettsia parkeri, which is an obligately intracellular bacterium, has across the American continents as infecting different tick species been reported and has been associated with a relatively mild human disease [1]. KH2PO4, 7.2 mM K2HPO4, and 3.9 mM glutamate monosodium [pH 7.0], filter sterilized), and the microorganism was isolated in Vero cells as described previously [2]. The sequences of gltA, sca0, and sca5 (GenBank accession numbers KJ158742, KJ158741, and KJ158744) showed 100% identity with R. parkeri strain Atlantic Rainforest [2]. R. parkeri strain Atlantic Rainforest was cultivated in 20 150-cm2 cell culture flasks containing Vero cell monolayers and released from the cells by sonication, pelleted by high-speed centrifugation, and purified by discontinuous renografin gradient ultracentrifugation [5, 6].

Results

Conclusion