Whole genome sequence based capsular typing and antimicrobial resistance prediction of Group B streptococcal isolates from colonized pregnant women in Nigeria

Mienye Bob-Manuel,Orikomaba K Obunge,Mary A Alex-Wele,Kennedy T Wariso,Jeremiah A Igunma,Lesley Mcgee

doi:10.1186/s12864-021-07929-z

Mienye Bob-Manuel, Orikomaba K Obunge + Show 4 more

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BackgroundStreptococcus agalactiae (Group B Streptococcus, GBS) is one of the major bacterial pathogens responsible for neonatal sepsis. Whole genome sequencing has, in recent years, emerged as a reliable tool for capsular typing and antimicrobial resistance prediction. This study characterized vaginal and rectal isolates of Group B Streptococcus obtained from pregnant women in Port Harcourt, Nigeria using a whole-genome sequence-based approach.ResultsCapsular types Ia, Ib, II, III, IV and V were detected among the 43 isolates sequenced. Twelve sequence types (STs) were identified, with ST19 (n = 9, 27.3 %) and ST486 (n = 5, 15.2 %) the most frequent among non-duplicated isolates. Of the alpha-like proteins (alp) identified, Alp1 was the most prevalent in 11 (33.3 %) isolates. Macrolide and lincosamide resistance determinants were present in 15 (45.5 %) isolates; ermB was detected in 1 (3 %), ermTR in 7 (21.2 %) isolates, lnu gene was detected in 6 (18.2 %) and mef was identified in 3 (9.1 %) isolates. Resistance of GBS to erythromycin and clindamycin (predicted from presence of erm or mef genes) was found to be 30.3 % and 24.2 %, respectively. All isolates were predicted resistant to tetracycline with only the tetM gene identified. Fluoroquinolone-resistance conferring substitutions in gyrA + parC were detected in 9 (27.3 %) isolates and chloramphenicol resistance was predicted from presence of aac6’-aph2 gene in 11 (33.3 %).ConclusionsThe data available from the whole genome sequencing of these isolates offers a small but insightful description of common serotypes and resistance features within colonizing GBS in Nigeria.

Highlights

Streptococcus agalactiae, known as Group B Streptococcus (GBS) is increasingly being recognized in Africa as one of the major pathogens responsible for neonatal sepsis [1,2,3]
The use of intrapartum antibiotic prophylaxis (IAP) may inadvertently result in the increase of antimicrobial resistance (AMR) [8]
This study reports the molecular characterization of colonizing strains of Group B Streptococcus in the vagina and rectum of pregnant women

Summary

Introduction

Streptococcus agalactiae, known as Group B Streptococcus (GBS) is increasingly being recognized in Africa as one of the major pathogens responsible for neonatal sepsis [1,2,3]. Some countries, including the United States and Australia, have policies regarding intrapartum antibiotic prophylaxis (IAP) to prevent early-onset disease (EOD) with resulting benefits [6, 7]. Whole genome sequencing (WGS) has emerged as a reliable and cost-effective method for capsular typing and for accurate predictions of minimum inhibitory concentrations for antimicrobials in GBS [13,14,15]. Streptococcus agalactiae (Group B Streptococcus, GBS) is one of the major bacterial pathogens responsible for neonatal sepsis. In recent years, emerged as a reliable tool for capsular typing and antimicrobial resistance prediction. This study characterized vaginal and rectal isolates of Group B Streptococcus obtained from pregnant women in Port Harcourt, Nigeria using a whole-genome sequence-based approach

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