Wharton jelly stem cells inhibits AGS gastric cancer cells through induction of apoptosis and modification of MAPK and NF-κB signaling pathways

Samaneh Abbasi,Reza Bazyar,Mohammad Ali Saremi,Gholamhoseen Alishiri,Nasrin Seyyedsani,Mahdieh Farhoudi Sefidan Jadid,Afshin Khorrami,Parisa Emami Golmarz,Gholamreza Jahangirzadeh,Ahmad Babazadeh Bedoustani,Alireza Isazadeh,Saba Hajazimian,Fatemeh Firouzi Amoodizaj

doi:10.1016/j.tice.2021.101597

Samaneh Abbasi, Reza Bazyar + Show 11 more

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https://doi.org/10.1016/j.tice.2021.101597

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BackgroundGastric cancer) GC) is one of the most common cancer with high mortality worldwide. The human Wharton's jelly stem cells (hWJSCs) can inhibit several cancer cells through several molecular pathways. Therefore, the present study aimed to investigate anticancer effects of hWJSCs conditioned medium (hWJSC-CM) and cell-free lysate (hWJSC-CL) against of GC cell line AGS and underlying signaling pathways. MethodsIn this study, we evaluated the effects of hWJSC-CM and hWJSC-CL on viability, proliferation, migration, invasion, apoptosis, and MAPK and NF-κB signaling pathways in AGS cells. Moreover, mRNA expression of genes involved in apoptosis (BAX, BCL2, SMAC, and SURVIVIN), as well as expression of proteins involved in NF-κB and MAPK signaling pathways were evaluated. ResultsThe obtained results showed that the hWJSC-CM and hWJSC-CL decreased viability, migration, and invasion of GC cell line AGS in a concentration and time dependent manner. We observed that the hWJSC-CM and hWJSC-CL induced apoptosis pathway through regulation of apoptosis involved genes mRNA expression. In addition, the hWJSC-CM and hWJSC-CL suppressed NF-κB signaling pathways as well as promoted MAPK signaling pathways. ConclusionIn general, our study suggested that the hWJSC-CM and hWJSC-CL inhibits proliferation and viability of GC cell line AGS through induction of apoptosis, as well as modification of NF-κB and MAPK signaling pathways.

Highlights

Gastric cancer (GC) is a prevalent cancer and third cause of cancer death worldwide [1]
In general, our study suggested that the human Wharton’s jelly stem cells (hWJSCs)-CM and hWJSC-CL inhibits proliferation and viability of GC cell line AGS through induction of apoptosis, as well as modification of NF-κB and MAPK signaling pathways
The cancer cells were treated with hWJSC-CM (44%) and hWJSC-CL (14%), and incubated in standard condition for 48 hours

Summary

Introduction

Gastric cancer (GC) is a prevalent cancer and third cause of cancer death worldwide [1]. Use of novel anticancer agents with natural source are required for treatment and prolonged survival of GC patients [4]. Stem cells therapy is an applicable and favorites approach in treatment of various human cancers. The hWJSCs are multipotent with high proliferative potential, and have mesenchymal and embryonic stem cell characteristics [6]. Previous studies reported that the hWJSCs have an anti-proliferative effects against several human cancer cells [8, 9]. Underlying mechanisms of the hWJSCs anticancer effects have not been identified. The human Wharton's jelly stem cells (hWJSCs) can inhibit several cancer cells through several molecular pathways. The present study aimed to investigate anticancer effects of hWJSCs conditioned medium (hWJSC-CM) and cell-free lysate (hWJSC-CL) against of GC cell line AGS and underlying signaling pathways

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