WebFARM: web server for finite automated restriction mapping

Tiratha Raj Singh

doi:10.6026/97320630004341

Abstract

Restriction endonucleases are indispensable tools in molecular biology and biotechnology. Type II restriction endonucleases are part of restriction modification systems. DNA fragment extraction and restriction mapping are the basis for several biotechnological activities. WebFARM is a server application for identifying restriction endonuclease recognition sites and to give information regarding restriction mapping for given nucleotide sequences. WebFARM analyses given nucleotide sequence and identify restriction site for selected restriction endonucleases. It will also provide frequency of restriction for each restriction endonuclease. http://webfarm.bioinfoindia.org/

Highlights

Restriction endonucleases (REnases) are part of restriction and modification (RM) systems, ubiquitous among bacterial DNA [1, 2]
RM systems were originally suggested to evolve as a defense mechanism against phage infection and other type of DNA invasion
The main criterion for the classification of type II REnases is their high specificity of cleavage within or close to their recognition site and they do not require ATP hydrolysis for their nucleolytic activity [5]

Summary

Introduction

Restriction endonucleases (REnases) are part of restriction and modification (RM) systems, ubiquitous among bacterial DNA [1, 2]. Background: Restriction endonucleases (REnases) are part of restriction and modification (RM) systems, ubiquitous among bacterial DNA [1, 2]. RM systems were originally suggested to evolve as a defense mechanism against phage infection and other type of DNA invasion. Type II REnases are intensely studied enzymes from the structurefunction perspective [3, 4].

Results

Conclusion