Weak magnetic carriers reduce nitrite accumulation and boost denitrification at high nitrate concentrations by enriching functional bacteria and enhancing electron transfer

Abstract

Biological denitrification is the dominant method for NO3− removal from wastewater, while high NO3− leads to NO2− accumulation and inhibits denitrification performance. In this study, different weak magnetic carriers (0, 0.3, 0.6, 0.9 mT) were used to enhance biological denitrification at NO3− of 50–2400 mg/L. The effect of magnetic carriers on the removal and mechanism of denitrification of high NO3− was investigated. The results showed that 0.6 and 0.9 mT carriers significantly enhanced the TN removal efficiency (>99%) and reduced the accumulation of NO2− (by > 97%) at NO3− of 1200–2400 mg/L 0.6 and 0.9 mT carriers stimulated microbial electron transport by improving the abundances of coenzyme Q-cytochrome C reductase (by 4.44–23.30%) and cytochrome C (by 2.90–16.77%), which contributed to the enhanced elimination of NO3− and NO2−. 0.6 and 0.9 mT carriers increased the activities of NAR (by 3.74–37.59%) and NIR (by 5.01–8.24%). The abundance of narG genes in 0.6 and 0.9 mT was 1.47–2.35 and 1.38–1.75 times that of R1, respectively, and the abundance of nirS genes was 1.49–2.83 and 1.55–2.39 times that of R1, respectively. Denitrifying microorganisms, e.g., Halomonas, Thauera and Pseudomonas were enriched at 0.6 and 0.9 mT carriers, which benefited to the advanced denitrification performance. This study suggests that weak magnetic carriers can help to enhance the biological denitrification of high NO3− wastewater.