Abstract
We present non-covalent, weak-affinity fluorophore labels for multi-target super-resolution microscopy. The working principle is that transient binding to and from a target, together with sequential imaging of multiple targets, circumvents the "spectral barrier" in fluorescence microscopy. We report on different types of weak affinity markers, their application in multi-target single-molecule and STED super-resolution microscopy, and demonstrate that intrinsically reduced photobleaching enables long-time super-resolution imaging in living cells.
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