WDP formulations using a novel mosquitocidal bacteria, Bacillus thuringiensis subsp. israelensis/tochigiensis (VCRC B-474) – Development and storage stability

Abstract

A novel mosquito active strain, Bacillus thuringiensis (VCRC B474) sharing the antigens of 2 serotypes, namely israelensis &tochigiensis was characterized by scanning electron microscopy and SDS-PAGE. The spherical and ovoid crystals present in this strain was composed of major polypeptides the size of 28, 65, and 130 kDa respectively. The sporulated cell mass was formulated into water dispersible powder (WDP) formulations with different carrier materials and checked for activity against Culex quinquefasciatus larvae at monthly intervals for up to a year. The formulation containing chalk was the most effective with LC50 values ranging between 0.274–0.523 μg/ml compared to the formulations containing bentonite (0.335-0.775) μg/ml and talc (0.348-0.808 μg/ml). The decline in the activity of these formulations with storage period was as follows: 3 months −14%, 22%, 20% respectively, 6 months - 25%, 35%, 37% respectively, 9 months − 39%, 50%, 47% respectively and 12 months −52%, 43%, 40% respectively. This study demonstrated that wet biomass of bacterial isolates could be simply mixed with carrier materials, dried and used for mosquito larval control without significant loss of activity for up to 6 months at room temperature. Further, this strain of Bacillus thuringiensis var. israelensis/tochigiensis (H14/19) can be a prospective candidate for use in mosquito control programs.