Abstract
In the Eastern Gangetic Plain of South Asia field pea (Pisum sativum L.) is often grown as a relay crop where soil waterlogging (WL) causes germination failure. To assess if selection for WL tolerance is feasible, we studied the response to WL stress at germination stage in a recombinant inbred line (RIL) population from a bi-parental cross between WL-contrasting parents and in a diversity panel to identify extreme phenotypes, understand the genetics of WL tolerance and find traits for possible use in indirect selection. The RIL population and the diversity panel were screened to test the ability of germination under both waterlogged and drained soils. A total of 50, most WL tolerant and sensitive, genotypes from each of both the RIL and the diversity panel were further evaluated to assay testa integrity/leakage in CaSO4 solution. Morphological characterization of both populations was undertaken. A wide range of variation in the ability to germination in waterlogged soil was observed in the RIL population (6–93%) and the diversity panel (5–100%) with a high broad-sense heritability (H2 > 85%). The variation was continuously distributed indicating polygenic control. Most genotypes with a dark colored testa (90%) were WL tolerant, whereas those with a light colored testa were all WL sensitive in both the RIL population and diversity panel. Testa integrity, measured by electrical conductivity of the leakage solute, was strongly associated with WL tolerance in the RIL population (rG = −1.00) and the diversity panel (rG = −0.90). Therefore, testa integrity can be effectively used in indirect selection for WL tolerance. Response to selection for WL tolerance at germination is confidently predicted enabling the adaptation of the ancient model pea to extreme precipitation events at germination.
Highlights
Peas (Pisum sativum L.) are an important pulse crop, ranks second in global production after beans among the pulse crops (Food and Agriculture Organization [FAO], 2017)
To assess if selection for WL tolerance is feasible in peas, we studied the response to WL stress at germination stage in a recombinant inbred line (RIL) population from a bi-parental cross between WL-contrasting parents and a diversity panel to: (i) identify extreme phenotypes for WL tolerance, (ii) understand the genetic basis of WL tolerance, and (iii) find traits for possible use in indirect selection for WL tolerance
A high broadsense heritability of H2 = 89% was found for germination under waterlogged conditions for this RIL population
Summary
Peas (Pisum sativum L.) are an important pulse crop, ranks second in global production after beans among the pulse crops (Food and Agriculture Organization [FAO], 2017). Pea seeds are rich in protein, slowly digestible starch, soluble sugars, fiber, minerals, and vitamins (Dahl et al, 2012). It has an economic and agronomic importance in cropping systems (Yang et al, 2018). The crop is Indirect Selection for Waterlogging Tolerance an important component of agroecological cropping systems in diverse regions of the world. Waterlogging (WL) can cause germination failure (Crawford, 1977) and lead to reduced plant population in pea (Zaman et al, 2018)
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