Abstract
Changes in levels of fluorescence substances (FS) in aqueous extracts of Citrus unshiu (C. unshiu) peels were measured by the 3-dimensional (3-D) fluorescence spectrophotometer. FS, extracted from C. unshiu peels, have characteristic fluorescence at excitation (Ex) 320-330 nm and emission (Em) 420-440 nm. Ex and Em of FS are very similar to those of lipofucsin-like-compounds known to accumulate in tissues during aging and fluorescent-chlorophyll-catabolites (FCC) appeared to be a product of oxidative cleavage of chlorophyll (Chl)-porphyrin. The amount of FS increased in yellow-green C. unshiu peels and decreased in yellow C. unshiu peels. In ethylene-treated C. unshiu peels, the amount of FS increased at the time of rapid disappearance of Chl, and decreased after that, whereas FS of non-treated C. unshiu peels did not change during incubation. A possible participation of FS in Chl catabolism is discussed.
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