Water Flow from Different Solutions During Perfusion of the Functioning Portion of the Small Intestine of the Rats In vivo

Abstract

Objective: The study of the water flow during perfusion of the functioning portion of the small intestine of male rats in vivo by Ringer solution, glucose and glycine. Methods: The experiments were performed on the functioning portion of the small intestine of the rats in the chronic experiments in vivo without narcosis, pain, stress and operation trauma, formed according method. The concentration of PEG was determined based on modified method colorimetrically on CFC-2MP, λ=465nm. The concentration of glycine was determined using method colorimetrically on photoelectrocolorimeter–CFC-2MP, λ=540 nm. The concentration of glucose was determined colorimetrically on photoelectrocolorimeter–CFC-2MP, λ=625 nm. The concentration of bile was determined colorimetrically on CFC-2MP, λ=480 nm. Results: There were no found the dilution of the perfusate with physiological fluids. There were detected an absorption of water from the Ringer solution, from the 25 mmol/L solutions of glucose or glycine, prepared on the Ringer solution. Water absorption from the Ringer's solution was more than from the solutions of 25 mmol/L glycine or glucose, prepared on the same Ringer's solution. It was shown the periodic character of the secretion of bile into the living small intestine of the rats under physiological conditions. Conclusion: Comparison of water flows in the unclosed functioning portion of the small intestine during the thereof perfusion by solutions of Ringer, glucose, or of equimolar concentration glycine showed that in all cases, regardless of the type of the substrate and presence of primordial physiological fluids in the intestine, water absorption is observed from the perfusion solution. Thus, it shows a high rate of water absorption in the small intestine, exceeding the dilution of the perfusion solution.