Waste Cooking Oil for Wax Esters Synthesis Using Candida Rugosa Lipase Immobilized on Fe3o4@oa Nanoparticles

Abstract

Wax ester is an important skin conditioning agent derived from the esterification of fatty acids with alcohols. It is widely used in cosmetic as it has a composition similar to that of natural skin lipids. This study presents the synthesis of wax esters from hydrolyzed WCO in presence of cetyl alcohol and Candida rugosa lipase immobilized on Fe3O4@OA. The effects of the reaction time and cycles of reuse were investigated. The wax ester conversion has been monitored for 24 h. High conversion of ~ 89.3 % was observed already at 12 hours of synthesis. No significant reduction, during four cycles of use, of the lipase activity occurs.