Abstract
The broad objective of this research is to study the effect of warm stratification on Cycas revoluta zygotic embryos length, seed germination and plant development. Four warm treatments were applied to seeds. Our results showed that seeds storage at room temperature or 30°C improved significantly zygotic embryos length. Moreover, time of germination was significantly reduced with the warm stratification. The highest percentage of germination was obtained with seeds warm treated at 30°C for 2 months while only 25% of seeds were able to germinate in the control. Regarding seedlings development, our results demonstrated that warm stratification did not affect plant development. No significant differences have been recorded in all the evaluated parameters except for root length. Taken together, these results underlined the beneficial effect of warm stratification on Cycas revoluta seed germination and plant development and proposed a new method to improve seed germination of Cycas revoluta.
Highlights
The sago palm (Cycas revoluta L.) is one of the widespread ornamental trees, grown in temperate, subtropical and tropical regions more precise ly in Miyazaki and Kagoshima Prefectures in Kyushu District down to the Ryukyu Islands, Okinawa Prefecture in Japan (Zarchini et al, 2011)
The broad objective of this research is to study the effect of warm stratification on Cycas revoluta zygotic embryos length, seed germination and plant development
Zygotic embryos (ZEs) length was investigated in the different warm pretreatments
Summary
The sago palm (Cycas revoluta L.) is one of the widespread ornamental trees, grown in temperate, subtropical and tropical regions more precise ly in Miyazaki and Kagoshima Prefectures in Kyushu District down to the Ryukyu Islands, Okinawa Prefecture in Japan (Zarchini et al, 2011). Described as one of the most primitive species among the living cycads, Cycas revoluta has been used as an indoor and outdoor landscape plant for centuries (Stevenson, 1990; Jones, 1994). Cycas revoluta is propagated either from seeds, which remain viable for only a short time, or from vegetative offshoots (Demiray et al, 2017). Germination of seed of Cycas revoluta is hard and time consuming (Zarchini et al, 2011). C. revoluta seeds demonstrates rapid loss of viability and low morphogenic potential, which hinder its conservation (NADERI et al, 2015)
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