Wamena Arabica Coffee (Coffea Arabica) Bean Extract Cream Inhibits The Increase Of Tyrosynase And Melanin Levels In Guinea Pig (Cavia Porcellus) Exposed To UVB Lights

Abstract

Introduction: Most of the aging process in the skin occurs due to exposure to sunlight, especially UV-B light, resulting in an increase in the activity of the tyrosinase enzyme and the amount of melanin which will cause hyperpigmentation of the skin. Antioxidants are substances that can provide protection from endogenous and exogenous oxidative stress by free radicals. The purpose of this study was to prove that Wamena roasted Arabica coffee bean extract cream can inhibit the enzymes tyrosinase and melanin in guinea pig exposed to UV-B lights. Methods: The design of this study was an animal experimental design with a randomized post-test only control group. The subjects of the study were 30 guinea pigs divided into 3 groups: group 1 as control, group 2 with placebo cream and group 3 was with 3% Wamena arabica coffee bean extract cream. The group 2 and 3 were exposed to UV-B light for 2 weeks. The skin of the guinea pig was excised for examination of the levels of the enzymes tyrosinase and melanin. Result: The average tyrosinase and melanin levels in control group were 19.891% and 4.62%, treatment group 2 was an average of 42.699% and 9.26% and treatment group 3 was 23.886% and 4.87%. Conclusion: Arabica coffee (Coffea arabica) bean extract cream roasted Wamena 3% inhibits the tyrosinase enzyme levels and inhibits the melanin in guinea pigs (Cavia porcellus) which are exposed to ultraviolet B light.