Abstract
carrier system in apical membrane. Phloretin, a GLUT2 inhibitor, significantly inhibited glucose uptake, especially at high glucose concentration and in incubation durations of >1 min raising the possibility that the increased Km and Vmax were due to translocation of GLUT2. Western blotting of the apical membrane using antibodies against SGLT-1 and GLUT2 suggested that GLUT2 rather than SGLT-1 translocated to apical membrane. Nocodazole inhibited carrier-mediated glucose uptake at high glucose concentrations (25 and 50 mM) during the 5 and 10 min incubation, but not at low glucose concentrations (0.5-10 mM) or with short durations of exposure (30 s and 1 min). CONCLUSION: GLUT2 is translocated to the intestinal apical membrane of Caco-2 cells via a glucose-dependent mechanism acting via the microtubular system. (Support: NIH DK39337-MGS)
Published Version
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