Voltammetry at a Hanging Mercury Drop Electrode as a Tool for the Study of the Interaction of Double‐stranded DNA with Genotoxic 4‐Nitrobiphenyl

Abstract

AbstractDifferential pulse voltammetry, cyclic voltammetry, alternating current voltammetry, and chronocoulometry at a hanging mercury drop electrode (HMDE) and DNA modified HMDE were used for the study of the interaction of 4‐nitrobiphenyl (4‐NBP) with double‐stranded DNA and for the detection of DNA damage caused by its interaction with 4‐NBP. Moreover, the interaction of DNA with intermediates of the 4‐NBP reduction and with 4‐aminobiphenyl, a metabolite of 4‐NBP, was investigated. The mechanism of the electrochemical reduction of 4‐NBP was investigated using CV, too. It was found that the reduction of 4‐NBP is controlled by both diffusion and adsorption. The rate constant of the reduction and the diffusion coefficient were calculated as well.