Abstract

A novel halovirus, VOLN27B, was isolated from a drill core sample taken at a depth of approximately 430 m, from a layer formed during the Cretaceous period (Anhui, China). VOLN27B infects the halophilic archaeon Halorubrum sp. LN27 and has a head-tailed morphotype with a contractile tail, typical of myoviruses. The average head diameter is 64 ± 2.0 nm, and uncontracted tails are 15 ± 1.0 × 65 ± 2.0 nm. The latent period is about 10 h. The maturing time of VOLN27B in cells of Halorubrum sp. LN27 was nearly 8 h. The adsorption time of VOLN27B on cells of Halorubrum sp. LN27 was less than 1 min. Virus particles are unstable at pH values less than 5 or when the NaCl concentration is below 12% (w/v). VOLN27B and Halorubrum sp. LN27 were recovered from the same hypersaline environment and provide a new virus-host system in haloarchaea.

Highlights

  • Hypersaline environments, near salt saturation, are commonly found to harbor members of the extremely halophilic archaea, which are often the dominant cellular microorganisms and can grow to high cell densities (>107 cells per milliliter)

  • Viruses infecting haloarchaea are typically observed to be in much higher concentration than their host cell population, and direct examination of natural brines shows a number of different morphological types of virus-like particles (VLP), including spherical, spindle-shaped, and head-tail [1,2,3,4]

  • Head-tailed VLPs are a less-frequent morphotype observed in hypersaline environments, they are commonly isolated and show great diversity [7]

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Summary

Introduction

Hypersaline environments, near salt saturation, are commonly found to harbor members of the extremely halophilic archaea (haloarchaea; Class Halobacteria), which are often the dominant cellular microorganisms and can grow to high cell densities (>107 cells per milliliter). Viruses infecting haloarchaea are typically observed to be in much higher concentration than their host cell population, and direct examination of natural brines shows a number of different morphological types of virus-like particles (VLP), including spherical, spindle-shaped, and head-tail [1,2,3,4]. In a comprehensive study of a salt lake in Senegal (Lake Retba), the morphotypes (and percentage) of VLPs were as follows: spindle-shaped/fusiform (43%), spherical (35%) particles, and head-tail (1%), while linear and various other, unusual morphological particles accounted for the remainder [3]. Head-tailed VLPs are a less-frequent morphotype observed in hypersaline environments, they are commonly isolated and show great diversity [7]. A brand new taxonomy of archaeal tailed viruses has been proposed that all genomesequenced archaeal tailed viruses were classified into three orders, i.e., Thumleimavirales, Kirjokansivirales, and Methanobavirales (https://talk.ictvonline.org/files/proposals/archaealviruses/m/ec-approved-awaiting-ictv-ratification)

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