Volatile Fatty Acids in Ruminal Fluid Can Be Used to Predict Methane Yield of Dairy Cows.

Abstract

Simple SummaryMethane emissions from cattle are difficult to measure, and some proxies used to estimate them have required information that is not always available. An example is predicting methane from the fatty-acid profile of milk, but this strategy is not suitable for non-lactating animals. We propose equations to predict the methane emitted per unit of feed eaten (methane yield) based on the volatile fatty acids within the rumen of the animals. Three of the seven equations we investigated were equally good at predicting the methane yield of dairy cows. Validation of these equations using previously published results indicated that the equations should also work for beef cattle. Being able to predict the methane yield for all classes of cattle means that a single strategy can be used, eliminating differences because of the use of different methods for different animal classes. Further work is necessary, but our strategy should be able to be adapted for use in cattle production environments. Being able to predict the methane of production animals will enable accurate estimates of the methane emissions from those animals, and assessment of strategies to reduce those emissions.The dry matter intake (DMI) of forage-fed cattle can be used to predict their methane emissions. However, many cattle are fed concentrate-rich diets that decrease their methane yield. A range of equations predicting methane yield exist, but most use information that is generally unavailable when animals are fed in groups or grazing. The aim of this research was to develop equations based on proportions of ruminal volatile-fatty-acids to predict methane yield of dairy cows fed forage-dominant as well as concentrate-rich diets. Data were collated from seven experiments with a total of 24 treatments, from 215 cows. Forage in the diets ranged from 440 to 1000 g/kg. Methane was measured either by open-circuit respiration chambers or a sulfur hexafluoride (SF6) technique. In all experiments, ruminal fluid was collected via the mouth approximately four hours after the start of feeding. Seven prediction equations were tested. Methane yield (MY) was equally best predicted by the following equations: MY = 4.08 × (acetate/propionate) + 7.05; MY = 3.28 × (acetate + butyrate)/propionate + 7.6; MY = 316/propionate + 4.4. These equations were validated against independent published data from both dairy and beef cattle consuming a wide range of diets. A concordance of 0.62 suggests these equations may be applicable for predicting methane yield from all cattle and not just dairy cows, with root mean-square error of prediction of 3.0 g CH4/kg dry matter intake.