Abstract
A Carotenoid Cleavage Dioxygenase (CCD) gene from Vitis vinifera was isolated and expressed in Escherichia coli . Recombinant VvCCD1 cleaved zeaxanthin symmetrically leading to the formation of 3-hydroxy-β-ionone, a C 13 -norisoprenoidic compound, and a C 14 -dialdehyde. Analysis of the gene expression during grape berries development revealed a significant induction of the gene before the onset of ripening, together with an increase in the level of C 13 -norisoprenoids throughout the maturity.
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