Abstract
Purpose: To discuss effects of vitexin glucolone (VG) to radiosensitivity of NSCLC (Non-small cell lung cancer) cell lines (A549 and H1299). Methods: Treating A549 and H1299 cells by VG with or without X-radiation. Cell viability was calculated by CCK8. Apoptosis rate was measured by flow cytometry and Western blot to expressions of protein. Subsequently, cells were transferred with TAK1 siRNA, cell viability and apoptosis were measured. Results: VG decreased the viability of Huh7 cells and inhibited effects of VG was more strengthen than radiotherapy in concentrations of 20 μmol/L and 40 μmol/L. Meanwhile, VG sensitized HCC exposed to radiation therapy to apoptosis as demonstrated by increased Bax/Bcl-2 ratio. In addition, VG enhanced the promotive effects of X-radiation on the expressions of TAK1, AMPKα1 and PPARγ. Furthermore, silence the expression of TAK1 partly reversed the effects of VG on HCC and radiosensitivity of NSCLC. Conclusion: VG enhances radiosensitivity of NSCLC via TAK1/AMPK pathway.
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