Abstract
Abstract This paper describes the possibility of a rapid and sensitive in vivo bioassay for assessing the endocrine-disrupting potential of effluent from wastewater treatment plants. The bioassay uses vitellogenin (Vg)-like gene expression in the liver of male zebrafish ( Danio rerio ). Adult male zebrafish were exposed to either 1 μ M carcinogenic 3-methylcholanthrene (3-MC) or 1 μ M 17 β -estradiol (E2) at 25°C for 48 h. The amplification of Vg-like mRNA by reverse transcriptase (RT) polymerase chain reaction (PCR) was then examined by using a newly designed primer pair and the total RNA extracted from the liver of the zebrafish. The results showed that 3-MC could induce vitellogenesis, as could E2. This suggests that this procedure is applicable as a rapid and sensitive in vivo bioassay. It also suggests that carcinogenic compounds such as 3-MC can disrupt the endocrine system.
Published Version
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