Abstract
The effect of the juvenile hormone analogue hydroprene on the appearance in the haemolymph of the yolk-protein precursor vitellogenin in male Diploptera punctata has been assessed using rocket immuno-electrophoresis. Vitellogenin was induced in a dose-dependent fashion, with a minimum dose of 10 μg hydroprene required for its appearance. Implantation of male corpora allata into male and female hosts also resulted in the appearance of vitellogenin in the haemolymph of the hosts, with females showing apparent greater sensitivity to the implantation. The identity of vitellogenin in male haemolymph was further confirmed using Ouchterlony double immunodiffusion and SDS polyacrylamide gel electrophoresis of immunoprecipitates. The electrophoretic pattern of immunoprecipitated haemolymph from females and hydroprene-treated males was similar, further confirming that the immunoprecipitable product was in fact vitellogenin.
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