Abstract
Vitellogenins (Vgs) and vitellins (Vns) were purified from reproductive female adult hemolymph (HL) and newly laid eggs of the bean bug, Riptortus clavatus. They were separated into two (Vg-1 and 2) and three (Vn-1′, Vn-1, and Vn-2) sub-components, respectively, by ion exchange chromatography. Fused rocket immunoelectrophoresis using anti-(Vn-1) serum showed that they are distinguished clearly into two immunologically distinct groups, i.e., Vg-1/Vn-1/Vn-1′ and Vg-2/Vn-2. SDS-PAGE analysis showed Vn-1 (Vn-1′) and Vn-2 have the same and/or smaller subunit component polypeptides as Vg-1 and Vg-2, respectively. Vn-1′ has some lower molecular weight peptides than Vn-1. Quantitative rocket immunoelectrophoretic analysis showed that Vg-1 and Vn-1 (plus V-1′) were detected first on day 3 in the HL and day 4 in the ovary, respectively, after adult emergence, and increased by day 10 in non-diapause female adults. Vg-1 synthesis is induced in diapause female adults in 1 day after the treatment with juvenile hormone (JH) and JH analog (JHA) or in a few days after transfer from short day to long day conditions. © 1996 Wiley-Liss, Inc.
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