Vitamin D3-stimulated bone formation in an organotypic ex vivo culture system of chick femora

Abstract

Event Abstract Back to Event Vitamin D3-stimulated bone formation in an organotypic ex vivo culture system of chick femora EL Smith1*, JM Kanczler1, CA Roberts1 and ROC Oreffo1 1 University of Southampton, Bone and Joint Research Group, United Kingdom Enhancement and application of our understanding of human skeletal developmental biology is critical to developing new tissue engineering approaches to skeletal repair. We propose that the use of the developing fetal femur to understand the differentiation of skeletal stem cells, skeletogenesis, and the effects of key differentiation agents, will aid our understanding of the developing bone and niche, providing a unique regenerative medicine paradigm. We have used a novel three dimensional organotypic culture system of embryonic chick femora, to investigate the effects of 1alpha,25-dihydroxyvitamin D3 on bone and cartilage development. {BR}Embryonic chick femurs from E11 were harvested and placed in organotypic cultures for 10 days in basal media alone, or basal media supplemented with 25uM vitamin D3. Organotypic cultures were analyzed by micro-computed tomography (microCT), and further assessed histologically for proteoglycan (alcian blue), collagen production (Sirius red), and mineralization (von Kossa). {BR}Stimulation of organotypic cultures with vitamin D3 increased the chick femur length (mm) by 17.5%, from E11(basal)=10.3±0.4 to E11(vitamin D3)=12.1±0.3. MicroCT analysis (10um resolution) demonstrated that, compared to basal cultures, stimulation with vitamin D3 increased Bone Volume/Total Volume (BV/TV) by 55.5%, increased Trabecular Thickness (TbTh (mm3)) by 33.3%, increased Trabecular Number (TbNo (mm)) by 18.6%, and decreased Trabecular Spacing (TbSp (mm)) by 16%. Histological analysis demonstrated an increase in bone formation in response to vitamin D3 stimulation, as evidenced by a 49% increase in collagen production, and increased bone mineralization as evidenced by a 20% increase in positive von Kossa calcium staining. {BR}This study demonstrates the successful use of organotypic chick femur cultures as a model system for bone development, evidenced by the effect of exogenous vitamin D3 to modulate bone formation. This organotypic model provides a tool for the temporal analysis of the key stages of bone and cartilage development, providing a paradigm for translation of bone development to improved scaffolds and skeletal stem cell treatments for regenerative medicine. Keywords: Bones, Bone Research Conference: 2011 joint meeting of the Bone Research Society & the British Orthopaedic Research Society, Cambridge, United Kingdom, 27 Jun - 29 Jun, 2011. Presentation Type: Poster Topic: Abstracts Citation: Smith E, Kanczler J, Roberts C and Oreffo R (2011). Vitamin D3-stimulated bone formation in an organotypic ex vivo culture system of chick femora. Front. Endocrinol. Conference Abstract: 2011 joint meeting of the Bone Research Society & the British Orthopaedic Research Society. doi: 10.3389/conf.fendo.2011.02.00062 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 30 Sep 2011; Published Online: 30 Sep 2011. * Correspondence: Dr. EL Smith, University of Southampton, Bone and Joint Research Group, Southampton, United Kingdom, E.L.Smith@soton.ac.uk Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers EL Smith JM Kanczler CA Roberts ROC Oreffo Google EL Smith JM Kanczler CA Roberts ROC Oreffo Google Scholar EL Smith JM Kanczler CA Roberts ROC Oreffo PubMed EL Smith JM Kanczler CA Roberts ROC Oreffo Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.