Abstract
Asthma oxidative stress disturbances seem to enable supplementary proinflammatory pathways, thus contributing to disease development and severity. The current study analyzed the impact of two types of oral vitamin D (VD) supplementation regimens on the redox balance using a murine model of acute ovalbumin-induced (OVA-induced) asthmatic inflammation. The experimental prevention group received a long-term daily dose of 50 µg/kg (total dose of 1300 µg/kg), whereas the rescue group underwent a short-term daily dose of 100 µg/kg (total dose of 400 µg/kg). The following oxidative stress parameters were analyzed in serum, bronchoalveolar lavage fluid (BALF) and lung tissue homogenate (LTH): total oxidative status, total antioxidant response, oxidative stress index, malondialdehyde and total thiols. Results showed that VD significantly reduced oxidative forces and increased the antioxidant capacity in the serum and LTH of treated mice. There was no statistically significant difference between the two types of VD supplementation. VD also exhibited an anti-inflammatory effect in all treated mice, reducing nitric oxide formation in serum and the expression of nuclear factor kappa B p65 in the lung. In conclusion, VD supplementation seems to exhibit a protective role in oxidative stress processes related to OVA-induced acute airway inflammation.
Highlights
The level of oxidative stress was increased in the experimental asthma group (ASTHMA), with total oxidative status (TOS), oxidative stress index (OSI) and malondialdehyde (MDA)
We observed that vitamin D (VD) therapy significantly improved the redox balance in treated asthmatic mice compared to non-treated asthmatic subjects
We found that total antioxidant response (TAR) was significantly decreased in the bronchoalveolar lavage fluid (BALF) and lung tissue homogenate (LTH) of asthmatic mice compared to healthy subjects
Summary
The prevalence of bronchial asthma [3] has been steadily increasing over the past years [4], notably in the pediatric segment [5], and many of these patients evolve into adults who have asthma [6]. Considering the significant burden generated by disease management and symptom control, considerable research has been conducted recently in the field of adjuvants for classical asthma therapy. One such topic evolves around the benefit of vitamin D (VD) supplementation, with more data required to support the intervention, as mentioned in the Global Initiative for Asthma Guideline [7]
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