Abstract
Background Deficiency vitamin D and vitamin D receptor (VDR) polymorphism are strongly associated with the susceptibility of tuberculosis in four seasons countries. As a country with sufficient sunlight, the incidence of tuberculosis in Indonesia remains high. Objective To asses the association between vitamin D serum levels and VDR FokI polymorphisms with the incidence of tuberculosis in children at Palembang. Methods A case control study was conducted at Department of Child Health Mohammad Hoesin Hospital Palembang on November 2011 to April 2012. Group samples consisted of children with tuberculosis based on inclusion criteria. Vitamin D (1.25(OH)2D3/calcitriol) serum level was measured by using IDS 1,25-Dihydroxy Vitamin D EIA kit and VDR FokI polymorphism was identified through RFLP analysis. Results Sixty subjects was divided equally to case and control groups. The mean of calcitriol serum level in case group was lower compared to control which are 105.5 (SD 66.9) pmol/L versus 162.9 (SD 52.9) pmol/L (P=0.001). We found nine subjects with calcitriol deficiency, eight out of thirty in children with tuberculosis and one out of thirty in healthy contacts (OR 10.5; 95% CI 1.2 to 90.7) pmol/L. The incidence of VDR FokI polymorphism was 28 out of 30 in case group and 22 out of 30 in control group (OR 5,0; 95% CI 0,9 to 26,4). Conclusion Vitamin D (calcitriol) deficiency and lower serum level are associated with higher risk of tuberculosis in children at Palembang. Polymorphism FokI in VDR gene also contributes to the susceptibility of tuberculosis.
Highlights
The aim of this study is to asses the association of vitamin D serum level and vitamin d receptor FokIpolymorphism with the incidence of tuberculosis in children at Palembang, Indonesia
We found nine subjects with calcitriol deficiency, 8 (26,7%) in children with tuberculosis disease and 1 (3,3%) in children without tuberculosis disease
The incidence of VDR FokIpolymorphism is 93,4% in case group and 73,3% in control group
Summary
Vitamin D serum levels and vitamin D receptor FokIpolymorphism on tuberculosis children in Palembang, Indonesia. Aim The role of vitamin D on host immunity defense against tuberculosis infection has long been known. Deficiency vitamin D and vitamin D receptor polymorphism are strongly associated with the susceptibility of tuberculosis in four seasons countries. As a country with sufficient sunlight, the incidence of tuberculosis in Indonesia remains high. The aim of this study is to asses the association of vitamin D serum level and vitamin d receptor FokIpolymorphism with the incidence of tuberculosis in children at Palembang, Indonesia. Method A case control study was conducted at Child Health Department Mohammad Hoesin Hospital Palembang during November 2011 - April 2012. Vitamin D (1,25(OH)2D3/calcitriol) serum level was measuredby using IDS 1,25-Dihydroxy Vitamin D EIAkit and VDR FokIpolymorphism was identified through RFLP analysis. A bivariate and multivariate analysis were performed with p < 0,05 and CI 95%
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