Abstract
Objective To study the vitamin D receptor (VDR) gene polymorphism in hyperthyroidism patients with osteoporosis, and to explore the impact of hyperthyroidism on bone metabolism in osteoporosis. Methods The whole blood samples of normal subjects (n=10, group A) and 10 patients with osteoporosis (group B) were obtained for determination of blood thyroid hormone (TSH) and free thyroid hormones (FT3 and FT4) levels using enzyme linked immunosorbent assay (ELISA). VDR gene was amplified, the fragment was recovered and digested by BsmⅠ, ApaⅠ, TaqⅠ and FoxkⅠ restriction enzymes, then polyacrylamide gel electrophoresis was performed to obtain VDR gene polymorphism maps. The VDR gene polymorphism was statistically analyzed. Results TSH levels were significantly lower, while FT3 and FT4 levels were significantly higher in group B than in group A. As compared with group A (3.70%), VDR gene in group B had significant polymorphism (83.43%). Conclusion Hyperthyroid can cause higher VDR gene mutation rate, resulting in bone metabolism imbalance, leading to osteoporosis. Key words: Hyperthyroidism; Osteoporosis; Vitamin D receptor; Gene polymorphism
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