Abstract
AGL15 (AGAMOUS-like 15) was originally identified as a sequence preferentially expressed in developing plant embryos by comparing PCR-generated ‘differential displays’ of embryo and non-embryo RNA populations. The sequence is expressed at low levels and encodes a member of the MADS domain family (named for the first four members to be identified: MCM1, AGAMOUS, DEFICIENS, and SERUM RESPONSE FACTOR) of regulatory factors. Members of the MADS domain family play pivotal roles in critical developmental events in a wide variety of organisms: in specification of mating type in yeast (MCM1), in responses to the environment in human cells (SRF), in control of mesoderm differentiation in Drosophila and muscle differentiation in humans (MEF2), and in determination of meristem and organ identity during flower development in plants (AGAMOUS, DEFICIENS/APETALA3, GLOBOSA/PISTILLATA, etc.). AGL15 is the only MADS domain factor identified to date that is expressed in a highly preferential manner in plant embryos.
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