Visualization of temporomandibular joint articular cartilage using synchrotron-radiation X-ray phase-contrast imaging

Abstract

X-ray phase-contrast imaging using synchrotron radiation was applied to visualize non-invasively the fine structures of articular cartilage of temporomandibular joint (TMJ). Among various X-ray phase-contrast imaging methods, we chose diffraction enhanced imaging (DEI) and carried out experiments using synchrotron radiation, aiming to visualize TMJ articular cartilage of rats and to identify the optimum X-ray energy. For comparison, the same sample was observed by a conventional laboratory X-ray source and X-ray micro-CT apparatus. DEI was able to visualize the articular cartilage of the mandibular condyle, which was not possible with the conventional laboratory X-ray source and X-ray micro-CT apparatus. DEI could also visualize the fibrous and proliferative layers in the articular cartilage of the mandibular condyle. We found that the optimum imaging energy was 20 keV for our sample. It was shown that DEI is a promising method for non-invasive visualization of TMJ articular cartilage. Our findings will open up new possibilities for applications of DEI with synchrotron radiation for basic medical and preclinical research.