Abstract
We have developed a novel autoradiographic method of visualizing oxygen fixation with sufficient delivery of [ 15 O ]O 2/O 2. Brain slices (400 μm) were preincubated in Krebs–Ringer phosphate buffer and exposed to [ 15 O ]O 2 in a chamber. Fixation of [ 15 O ]O 2 correlated with the polarographically measured oxygen consumption among tissue slices from various organs ( r=0.84). The fixation of [ 15 O ]O 2 by brain slices was significantly reduced (7.2% of the control) by heat-treatment or dose dependently by NaCN (18.2% of the control on 50 mM NaCN pretreatment). The 15 O radioactivity in the brain slices prepared from rotenone injected rats was also reduced compared to the control (56.8% of the control side). In an autoradiographic study, 15 O radioactivity showed a heterogeneous distribution both in coronal and sagittal sections. Autoradiography of young and senescent rat brain sections showed reduction of oxygen uptake with aging in the cerebrum, the senescent being 77.4% of the young. This method provides information regarding basic oxygen consumption of tissue slices under condition of sufficient O 2 delivery, which reflects mitochondrial electron transport.
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