Abstract
We have developed a novel in vitro autoradiographic method of visualizing oxygen fixation using [ 15 O ]O 2. Brain slices (400 μm) are preincubated in Krebs–Ringer phosphate buffer and exposed to [ 15 O ]O 2 with carrier O 2 for 10 min on slide glass or on a Millicell-CM membrane in a chamber. After the 15 O 2 exposure, the brain slices are placed in contact with an imaging plate. The autoradiograms are analyzed with MacBas to obtain images of the distribution of radioactivity. The [ 15 O ]O 2 fixation was more extensive in the gray matter (striatum, thalamus and cerebral cortex) than in the white matter. The fixation of [ 15 O ]O 2 in brain slices decreased dose dependently with NaCN concentration. These results indicate that [ 15 O ]O 2 is converted into [ 15 O ]H 2O in the brain tissue according to the activity of mitochondrial electron transfer and remains in the tissue. This method provides information regarding the basic oxygen consumption of brain slices of rat and mouse under conditions of sufficient O 2 delivery, which reflects mitochondrial electron transport. Themes: Cellular and Molecular Biology Topics: Staining, Tracing, and imaging techniques
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
