Visualization of degradation of injectable thermosensitive hydroxypropyl chitin modified by aggregation-induced emission

Abstract

The reversible thermosensitive hydroxypropyl chitin (HPCH) has been widely investigated for drug delivery, tissue engineering, wound repair and antibacterial hemostasis. To achieve non-invasive and real-time visualization of in vivo degradation of HPCH, aggregation-induced emission fluorogen (AIEgen) was introduced covalently in HPCH in water to obtain thermosensitive fluorescent hydroxypropyl chitin (FHPCH) avoiding using any organic solvent. The obtained fluorescent FHPCH hydrogel showed strong yellow-green fluorescence feature under UV irradiation, enabling visualization of the hydrogel with reversible thermosensitivity and reliable injectability. The in vitro enzymatic degradation study of FHPCH hydrogel showed that the attenuation of fluorescence intensity well matched with hydrogel weight loss. The injection through a 26-gauge needle for real-time fluorescence imaging in mice indeed indicated that AIE modified injectable thermosensitive FHPCH can be used for non-invasive, continuous and real-time visualization and quantitative analysis of in vivo degradation. This provides a new method for non-invasive real-time monitoring of similar implantable materials.