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Abstract
Tobacco mosaic virus RNA encapsulated in large unilamellar vesicles of phosphatidylserine was found to infect efficiently tobacco protoplasts prepared from suspension culture cells. Multiplication of tobacco mosaic virus in infected cells was confirmed by fluorescent antibody staining, electron microscopic observation, and infectivity assay. Virus infectivity was first detected at 6 hr after infection, it then increased rapidly and reached a plateau at 20 to 24 hr.
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