Abstract
Blasticidin S (BcS) and puromycin (PM), inhibitors against protein synthesis in fungi and higher organisms, inhibited tobacco mosaic virus (TMV) multiplication in tobacco leaf-discs, whereas chloramphenicol (CM), inhibitor against bacterial protein synthesis, did not inhibit. TMV produced by antibiotics treatment had the same infectivity as that produced by untreatment. BcS and PM inhibited 14C-leucine incorporation into the cytoplasmic protein of tobacco leaf-discs, but CM did not. CM reduced more rapidly the chlorophyll content in leaf-discs than BcS and PM. 14CO2-incorporation into infected leaf-discs was strongly inhibited by CM, but not by BcS and PM. Although, under BcS and PM treatments, the rate of 14C-amino acids and 14CO2-incorporations into TMV was in proportion to the amount of TMV produced, the rate of the former incorporation rather increased by CM under both light and dark conditions and that of 14CO2-incorporation was decreased, whereas the amount of TMV was not altered by CM. Most of 14CO2 incorporated into TMV located in TMV protein. Site of TMV synthesis in tobacco leaf-discs and the role of photosynthetic activity of chloroplasts are discussed.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
