Abstract
ABSTRACTFormycin B (FMB), when applied for 5–6 hr immediately after inoculation, inhibited tobacco mosaic virus (TMV) multiplication in tobacco leaf‐discs and local lesion formation on bean leaves. The inhibitory activity of FMB on local lesion formation was reversed by the addition of adenine, adenosine, and adenylic acid of 5 times in molar concentration as much as FMB, but not by hypoxanthine or uracil. FMB‐treatment did not cause significant changes in total RNA contents and in the synthetic rates in TMV‐inoculated and noninoculated tobacco leaf‐discs. However, 32P incorporation rates into TMV and the amount of TMV synthesized in leaf‐discs were remarkably reduced by FMB‐treatment. 32P‐phosphorylated FMB could not be detected either from FMB‐treated tobacco leaf RNA or from TMV‐RNA by means of column chromatography. 3H‐labeled FMB could not be detected in either TMV or in tobacco leaf RNA. The inhibitory action of FMB against TMV multiplication in tobacco leaf‐discs is discussed.
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