Abstract
Protocols were developed to eliminate viruses from vegetatively maintained groundnuts. The culture of shoot meristems was effective for virus elimination from interspecific Arachis hybrids. Peanut mottle potyvirus (PMV), peanut stripe potyvirus (PStV) and tomato spotted wilt tospovirus (TSWV) were not detected by DAS-ELISA in any plants regenerated from meristems treated with thermotherapy, alone or combined with chemotherapy. Only 2.0% of the plants regenerated from untreated meristems contained PMV, and none contained PStV or TSWV. Shoot tip culture was not as effective as meristem culture for elimination of PMV. Plants regenerated from untreated shoot tips (1 cm long) of Arachis hybrids remained infected with PMV, whereas 38% of the plants regenerated from shoot tips treated with thermotherapy plus chemotherapy were infected with PMV. Arachis hybrids were more readily freed of TSWV and PStV than of PMV. Meristem culture, thermotherapy and chemotherapy were not required for elimination of TSWV and PStV, and plants regenerated from untreated shoot tips were free of these viruses
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