Abstract
A direct antigen-coated indirect ELISA (ELISA-DAC) reliably detected peanut stripe potyvirus (PStV) in groundnut seed. PStV was detected in the cotyledons and embryo of all 90 infected seeds and in the testa of 2 seeds collected before harvest. The rate of PStV seed transmission was affected by groundnut genotype and length of infection. Incidence of PStV seed transmission varied from <1 to 50% in the 935 germplasm lines tested. The majority has seed transmission rates ranging from 5 to 20%. Groundnut cultivars and lines of the Spanish type showed higher seed transmission rates than those of other types. Several low seed transmission lines (<1% seed transmission) were obtained. PStV was detected in embryos of immature seeds at nearly the same percentage during pod development. Frequency of seed transmission varied according to the age of plants when they became infected. PStV transmission was higher in small than in large seeds.
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